NGERE is equipped with a mass spectrometer (Shimadzu LCMS 8045, LC-MS/MS ESI triple quad) enabling the simultaneous analysis of numerous molecules involved in various metabolic pathways such as energy metabolism, monocarbon metabolism, organic acid metabolism, etc. In order to broaden its spectrum of analysis, we are also equipped with a complementary module, the DPiMS, enabling metabolomic analysis from raw biopsies or histological sections normally incompatible with this type of instrument. This device enables us to routinely analyze and quantify various constituents in order to establish metabolic phenotyping on our cellular or animal models, or to study patient cohorts from plasma samples.

To complete this metabolic phenotyping, we also have a range of high-tech instruments at our disposal, such as a high-resolution oxygraph for in vivo measurement of mitochondrial respiration (Oroboros O2k), a spectral scanning microplate reader equipped with automated injectors for absorbance, luminescence and fluorescence detection (Molecular devices iD3) and an HPLC chain (Dionex, Ultimate 3000) needed to establish metabolic profiles on all our study models.

The use of animals for scientific purposes remains essential for the understanding of pathophysiological mechanisms and the search for new therapies for degenerative diseases. Our project aims to understand the molecular mechanisms linking disorders of monocarbon metabolism to degenerative pathologies of the liver, heart and brain, as well as the mechanisms involving the gut microbiota in the onset and evolution of chronic inflammatory bowel diseases. Our various experimental rodent models enable us to conduct behavioral, physiological and pharmacodynamic studies.

In addition to an animal facility which houses our animals in compliance with current ethical rules, our laboratory has both the equipment and the skills to explore the functional characteristics of our animals, thanks to very specific instruments such as the AIDA (Karl Storz) endoscope needed to establish a diagnosis by colorectal endoscopy, or an HR camera coupled with ViewPoint (videotracking) software dedicated to the acquisition of data generated during neurobehavioral tests.