Services offered by the Biophysics & Structural Biology Facility (B2S)

The Biophysics & Structural Biology facility offers the following services:

• Characterization of proteins and complexes in solution: Quality control measurements and functional analysis of protein samples (VP-DSC MicroCal, CD Chirascan-plus Applied Photophysics, DLS Malvern NanoSizer, NMR 600Mhz Bruker Avance III equipped with a TCI cryoprobe).
• Characterization of protein-protein and protein-ligand interactions:  Determination of kinetic and thermodynamic parameters of interactions in solution or not, directly or indirectly, by microcalorimetry (VP-iTC MicroCal, iTC200 MicroCal), surface plasmon resonance (Biacore X100 GE Healthcare), fluorescence or NMR approaches.
• Isolation and characterization of various compounds by liquid chromatography (Ultimate 3000 BioRS Thermo Fisher Scientific): Determination of the purity of a sample; identification, separation and monitoring of reactions, determination of compounds in a mixture.
• Structural characterization of proteins and complexes: Determination of the three-dimensional structure of biological macromolecules, their dynamics and their interaction mode by NMR or X-ray diffraction (TTPLAb Tech Mosquito, Diffractometer Bruker X8-Proteum).
• Fast kinetics: Characterization of very fast reactions, crucial step in the fine understanding of enzymatic mechanisms (Stopped-flows Applied Photophysics in absorbance, fluorescence or CD, quench-flow Kin Tek RQF-3).

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Services offered by the Flow Cytometry Facility

The Flow Cytometry facility offers the following services:

• Cellular tris.
• Flow cytometry analysis.
• Training in acquisition, analysis and interpretation of the results obtained.
• Provision of cytometers after validation of the training.
• Data analysis.
• Advice to users.

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Services offered by the Epitranscriptomics & Sequencing Facility (EpiRNA-Seq)

The Epitranscriptomics & Sequencing facility offers the following services:

• Epitranscriptome / Analyses of RNA modifications: The core facility is specialized in analysis of RNA, and, more specifically in RNA modifications. We are actively developing approaches based on specific treatment of RNA by selected chemical reagents, library preparation from treated RNA, sequencing and associated bioinformatic analysis.
• Transcriptome / RNA-Seq: The core facility offers you a transcriptomics service. The transcriptome is defined as a set of transcripts present in a cell at a given time and under defined conditions. The RNA-seq, using next-generation sequencing, is now a technique of choice proposed by the core facility that allows the global analysis of differential gene expression, alternative splicing and variations of non-coding RNAs (miRNA, lncRNA, etc).
• Sample Preparation / RNA extraction and quality control: The core facility can take care of RNA extraction and enrichment of your samples according to your projects and can perform quality control, in order to obtain optimal results for all your sequencing projects.
• Genomic DNA or exome sequencing: The core facility offers a DNA sequencing service (bacterial or eukaryotic genomic DNA or human exomes with prior enrichment).

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Services offered by the Functional Genomics Facility

The Functional Genomics facility offers the following services:

• Infinium HD genotyping: The Infinium HD assay (Illumina) allows genotyping studies and/or copy number variation (CNV) analyses. The technique allows, depending on the type of chip, the genotyping of SNPs from 3000 to more than 5 million loci in different species.
• High-throughput sequencing (NGS): The platform offers high-throughput DNA sequencing services using Illumina technology. A scientific and technical support for the choice of the library preparation strategy best adapted to the needs of the users is proposed (panels of targeted genes, sequencing of whole genomes (micro-organisms and soon whole genomes of higher organisms).
• Study of human genome methylation on a microarray: Genome-wide analysis of human DNA methylation is performed on the Infinium Methylation EPIC microarray (Illumina). This analysis allows the quantitative measurement of cytosine methylation of more than 850,000 CpG sites using a multiplex genotyping assay on sodium bisulfite-converted genomic DNA (gDNA). The processing scale is 8 samples per microarray.

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Services offered by the Imaging Facility (PTIBC)

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Services offered by the Proteomics Facility

The Proteomics facility offers the following services:

• LC-MALDI : Chemical and enzymatic treatment of samples, being either polyacrylamide gel pieces or water-based solutions (with restrictions regarding buffer contents), for exhaustive identification of protein content ; nanoHPLC fractionation ; MALDI MS and MS/MS analysis ; database interrogation and analytical report.
• Label-free relative quantification by LC-MALDI : Chemical and enzymatic treatment of samples, being either polyacrylamide gel pieces or water-based solutions (with restrictions regarding buffer contents), for exhaustive identification of protein content ; nanoHPLC fractionation ; MALDI MS and MS/MS analysis ; database interrogation and analytical report.
• Protein identification from polyacrylamide gel pieces : Chemical and enzymatic treatment of polyacrylamide gel pieces for identification of major protein content ; MALDI MS and MS/MS analysis ; database interrogation and analytical report.
• Analytical development: For specific needs, the platform develops dedicated protocols based on mono- or bi-dimensional protein electrophoresis, any HPLC method and mass spectrometry.

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