Research unit CITHEFOR
Cibles thérapeutiques, formulation et expertise préclinique du médicament
Bâtiment AB, Campus Brabois-Santé
9 Avenue de la Forêt de Haye, BP 20199
54505 VANDOEUVRE-LES-NANCY
FRANCE

+33.3.72.74.73.04
Director
Ariane BOUDIER
ariane.boudier [at] univ-lorraine.fr
Administrative Manager
Nathalie DEGOUSEE
nathalie.degousee [at] univ-lorraine.fr
UNIT'S WEBSITE
Drug, Nanoparticles, Microparticles, Quality control, Cell culture, Preclinical trial, Vasoreactivity, Redox signaling, Nitric Oxide

The UR CITHEFOR develops new therapeutic strategies for vascular diseases (treatment of stroke, prevention of atherosclerosis and restenosis). Its strategy is based on the sustained and controlled release (physiological concentration) of nitrogen monoxide (NO), to optimize its beneficial protective effects at the vascular level.

CITHEFOR thus synthesizes and ensures quality control, according to regulatory standards, of NO donors of the S-nitrosothiols (RSNO) type, physiological or derived from innovative compounds. These molecules have a known degree of purity and pharmaceutical quality. The control of NO release is optimized, depending on the route of administration, using particulate formulations (nano or micro) or nanostructured materials.

The pharmacological effects, including the identification of new targets as well as the modulation of redox signaling pathways, induced by free or formulated NO donors are evaluated using models (enzymatic, cellular, tissue and animal).

CITHEFOR is interested in the modulation of the regulatory pathways of vascular function through S-nitrosation of proteins (addition of NO to a thiol function) in connection with redox signaling.

Our expertise makes it possible to establish and study the connections between the antioxidant properties of redox molecules and the NO-dependent signaling pathways.

Supervising body(ies)

Key numbers of the unit

  • 15 Lecturers and professor
  • - Staff scientists
  • 8 Qualified research supervisors (HDR)
  • 5Technical and administrative staff
  • 9phD students
  • 103 Publications ACL
    2015-2020

In addition to its staff, the unit welcomes 3 persons in emeritus.

Research topics / Know-how

  • Thématique 1 Labo

    Innovative molecules and nanoformulations from nitric oxide donors

    The unit is a single multidisciplinary team and is articulated in a single project around the following disciplines: pharmacology, cell biology, galenic pharmacy, physicochemistry and analytical chemistry.

    This project addresses two major scientific questions to position nitric oxide (NO) donors of the S-nitrosothiol (RSNO) type in vascular pathologies: the control of NO release and the understanding of the induced signaling. These questions are intimately interconnected since to understand the molecular, cellular and tissue mechanisms involved, it is necessary to obtain a prolonged and controlled release of NO (in the nanomolar range). This can only be achieved by using original formulations.

    This project is part of the drug development process in coherence with the teaching affiliation at the Faculty of Pharmacy of all the teachers-researchers of the unit.

    Contact(s) :

    Ariane BOUDIER
    ariane.boudier [at] univ-lorraine.fr

Staff

  • Ariane BOUDIER
    Ariane BOUDIER Director UL
  • Avatar
    Igor CLAROT Co Director UL
  • AVATAR
    Nathalie DEGOUSEE Administrative Manager UL

phD students

Pas de soutenance pour le moment

  • 22/11/2023
    h00
    Amphithéâtre Gallé
    En savoir plus
    CELINE DELAITRE
    Caractérisation pharmacologique et fonctionnelle de ligands traceurs du récepteur AT1 de l'angiotensine II pour l'étude de nouvelles approches thérapeutiques
    Pharmacological and functional characterization of angiotensin II AT1 receptor tracer ligands for the study of new therapeutic approaches
    Directeur de thèse :
    François DUPUIS
    Co-directeur de thèse :
    Sandra LECAT
  • 24/10/2023
    13h30
    Salle de réunion J80.7
    En savoir plus
    CELIA BOUKOUFI
    Caractérisation des propriétés catalytiques de nanoparticules d'or immobilisées
    Characterization of the catalytic properties of immobilized gold nanoparticles
    Directeur de thèse :
    Igor CLAROT
    Co-directeur de thèse :
    Ariane BOUDIER
  • 20/09/2022
    9h00
    amphi Lepois
    En savoir plus
    MARGAUX BERTHOU
    MONITORING DE S-NITROSOTHIOLS DANS DES MATRICES BIOLOGIQUES GRACE A DES SONDES NANOSTRUCTUREES
    S-NITROSOTHIOLS MONITORING IN BIOLOGICAL MATRICES BY NANOSTRUCTURATED PROBES.
    Directeur de thèse :
    Igor CLAROT
    Co-directeur de thèse :
    Arnaud PALOTTA
  • 03/11/2021
    14h00
    Salle de réunion CITHEFOR
    En savoir plus
    JUSTINE BONETTI
    Potentiel thérapeutique des S-nitrosothiols dans la prévention de l’athérosclérose : Modulation de la métaplasie des monocytes et cellules musculaires lisses en cellules spumeuses
    Therapeutic potential of S-nitrosothiols in the prevention of atherosclerosis: Modulation of monocytes and smooth muscle cells metaplasia into foam cells
    Directeur de thèse :
    Caroline GAUCHER
    Co-directeur de thèse :
    Alfonso POMPELLA
    Co-tutelle :
    Università di SIENA
    ITALIE
  • 17/09/2021
    9h30
    Salle de réunion
    En savoir plus
    JORDAN BEURTON
    Optimisation physicochimique de films fonctionnalisés pour des applications cardio-vasculaires
    Physicochemical optimization of functionalized films for cardiovascular applications
    Directeur de thèse :
    Ariane BOUDIER
    Co-directeur de thèse :
    Philippe LAVALLE
  • 13/11/2019
    10h00
    Salle Gallé
    En savoir plus
    Romain SCHMITT
    S-nitrosoglutathion et perméabilité intestinale : de la mise en place d’un modèle de chambre de Ussing à l’étude de l’impact de ce donneur de monoxyde d’azote sur la barrière intestinale
    S-nitrosoglutathione and intestinal permeability: from the implementation of a Ussing chamber model to the study of the impact from this nitric oxide donor on the intestinal barrier
    Directeur de thèse :
    Anne SAPIN-MINET
  • 17/10/2019
    14h00
    salle de réunion
    En savoir plus
    Yi ZHOU
    Mécanismes de franchissement de la barrière intestinale et de stockage vasculaire des S-nitrosothiols pour l'amélioration de formulations orales de NO
    Mechanisms of S-nitrosothiols intestinal permeability and NO store formation within vascular wall to improve NO oral delivery systems
    Directeur de thèse :
    Caroline GAUCHER
    Co-directeur de thèse :
    Marianne PARENT

Equipment

The unit has many analytical, experimental and formulation tools that allow in particular:

  • To develop innovative formulations,
  • To characterize the formulations,
  • To quantify the substances of interest in biological media,
  • To evaluate their cellular toxicity,
  • To observe a potential pharmacological effect.

Services

  • Service 1

    Histological platform

    This platform allows the histological and morphometric study of tissues (paraffin, cryosections), cells (fixed or living) or polymer particles. The microscopic analyzes are carried out by optical microscopy with white light (conventional, phase contrast or polarized) or fluorescence.

    Contact(s) :

    Caroline SARRADO
    caroline.sarrado [at] univ-lorraine.fr

  • Service 2

    Pharmaceutical technology

    This platform allows preparation and characterization of pharmaceutical dosage forms. Technics apply more particularly to biodegradable formulations such as nano-, microparticules and implants…

    Contact(s) :

    Anne SAPIN-MINET
    anne.sapin [at] univ-lorraine.fr

  • Service 3

    Physicochemistry platform

    The physico-chemistry platform offers spectrophotometric (UV-Vis, fluorescence) and separation (HPLC and capillary electrophoresis) analytical tools necessary for the development of analytical and bioanalytical methods for the evaluation of drug candidates and innovative formulations (nano and micro- particulates).

    Contact(s) :

    Igor CLAROT
    igor.clarot [at] univ-lorraine.fr

  • Service 4

    Preclinical expertise

    Preclinical expertise (telemetry, isolated organs: arteries, intestines, etc.) is outsourced to the Animal Center Campus Brabois-Santé: pharmacodynamics, mechanisms of action, pharmacokinetics, identification of therapeutic targets (nitrosation of proteins, including receptors for angiotensin II).

    Contact(s) :

    François DUPUIS
    francois.dupuis [at] univ-lorraine.fr

  • Service 5

    Cell biology platform

    The cell / molecular biology platform offers models of stress (oxidant, ferroptosis), inflammation, wound healing and intestinal barrier for the evaluation of the mechanism of action (redox biomarkers, gene expression, protein identification) of drug candidates and their formulations.

    Contact(s) :

    Caroline GAUCHER
    caroline.gaucher [at] univ-lorraine.fr

Innovations

  • - Patents
     
  • 2 Invention declarations
     
  • - ERC grant
     

 

Publications

Articles in peer reviewed journals

All publications